SV40T 介导的人胎儿永生化气管成纤维细胞系的建立

doi:10.3969/j.issn.1004-616x.1999.01.008

Abstract

Abstract: The aim is immortalizing the human fetal t racheal fibroblast cells to study the cell neoplastic transformation. The primary human fetal t racheal fibroblast cell was t ransfected with pZIPSV40T vector. The colonies that resistant to G418 were collected , and passaged to immortalize. The biological characters immortalized cell had been determined. Result : the immortalized cell morphology is shorter than normal , arrange un2regular. The cells grow well at p50. Southern blot confirmed the presence of T antigen as integrated form in immortalized cell DNA. The index of growth period was fourth day after plated. All the cells could not form colony in soft agar.No tumors formed after SC injection of the cells in nude mice. The result confirmed that human fetal t racheal fibroblast cell can be immortalized by SV40T gene.

Key words: Immortalization, Human fetal fibroblast cell, Transformation

Yang Meiying, Ye Changqing, Liu Leihua. ESTABLISHMENT OF HUMAN FETAL TRACHEAL FIBROBLAST CELL LINE IMMORTALIZED BY SV40T[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(1): 22-024.

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ESTABLISHMENT OF HUMAN FETAL TRACHEAL FIBROBLAST CELL LINE IMMORTALIZED BY SV40T

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